Stabilized oil-in-water emulsions including meptyl dinocap

ABSTRACT

The present invention relates to stable, agricultural oil-in-water emulsion compositions.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional PatentApplication Ser. No. 61/063,492, filed Feb. 4, 2008, and U.S.Provisional Patent Application Ser. No. 61/068,529, filed Mar. 7, 2008,both of which are expressly incorporated by reference herein.

FIELD OF THE INVENTION

The present invention relates to stable, agricultural oil-in-wateremulsion compositions.

BACKGROUND AND SUMMARY

Concentrated oil-in water emulsions of liquid active ingredients oractive ingredients dissolved in a solvent are commonly used inagricultural compositions due to certain advantages provided over otherformulation types. Emulsions are water based, contain little or nosolvent, allow mixtures of active ingredients to be combined into asingle formulation and are compatible with a wide range of packagingmaterial. However, there are also several disadvantages of suchagricultural emulsions, namely that they are often complex formulationswhich require high amounts of surface-active agents for stabilization,are generally very viscous, have a tendency for Oswald ripening of theemulsion globules and separate over time. Therefore, improvements insuch emulsion formulations are needed in the agricultural field.

Several oil-in-water emulsion compositions for cosmetics anddermatological applications have been described in U.S. Pat. No.5,658,575; U.S. Pat. No. 5,925,364; U.S. Pat. No. 5,753,241; U.S. Pat.No. 5,925,341; U.S. Pat. No. 6,066,328; U.S. Pat. No. 6,120,778; U.S.Pat. No. 6,126,948; U.S. Pat. No. 6,689,371; U.S. Pat. No. 6,419,946;U.S. Pat. No. 6,541,018; U.S. Pat. No. 6,335,022; U.S. Pat. No.6,274,150; U.S. Pat. No. 6,375,960; U.S. Pat. No. 6,464,990; U.S. Pat.No. 6,413,527; U.S. Pat. Nos. 6,461,625; and 6,902,737; all of which areexpressly incorporated herein by reference. However, although thesetypes of emulsions have found advantageous use in personal careproducts, these types of emulsions have not been used previously withagriculturally active compounds, which are typically present inemulsions at much higher levels than cosmetic active ingredients.

One example of an agricultural oil-in-water emulsion composition that issuitable for agriculturally active ingredients that are liquid orsoluble in suitable solvents at relevant storage temperatures isdisclosed in U.S. patent application Ser. No. 11/495,228, the disclosureof which is expressly incorporated by reference herein.

The present invention is related to agricultural compositions comprisingan oil-in-water emulsion, the oil-in-water emulsion composition havingan oil phase and water phase, the oil-in-water emulsion compositioncomprising an oil adapted to form oily globules having a mean particlediameter of less than 800 nanometers, a polymeric modifier that iscompatible with the oil phase, at least one agriculturally activecompound, at least one non-ionic lipophilic surface-active agent, atleast one non-ionic hydrophilic surface-active agent, at least one ionicsurface-active agent, and water.

DETAILED DESCRIPTION

One embodiment of the present invention is a novel oil-in-water emulsioncomposition having an oil phase and water phase, the oil-in-wateremulsion composition comprising:

an oil adapted to form oily globules having a mean particle diameter ofless than 800 nanometers;

a polymeric modifier being compatible with the oil phase;

an agriculturally active ingredient of Meptyl Dinocap;

at least one non-ionic lipophilic surface-active agent,

at least one non-ionic hydrophilic surface-active agent;

at least one ionic surface-active agent; and

water.

The oil phase of the oil-in-water emulsion of the present inventionutilizes either Meptyl Dinocap which is in the form of an oil, oralternatively, an agriculturally active compound dissolved or mixed inan oil, to form the oily globules. An oil is by definition, a liquidwhich is not miscible with water. Any oil which is compatible with theagriculturally active compound may be used in the oil-in-water emulsionsof the present invention. The term ‘compatible’ means that the oil willdissolve or mix uniformly with the agriculturally active compound andallow for the formation of the oily globules of the oil-in-wateremulsion of the present invention. Exemplary oils include, but are notlimited to short-chain fatty acid triglycerides, silicone oils,petroleum fractions or hydrocarbons such as heavy aromatic naphthasolvents, light aromatic naphtha solvents, hydrotreated light petroleumdistillates, paraffinic solvents, mineral oil, alkylbenzenes, paraffinicoils, and the like; vegetable oils such as soy oil, rape seed oil,coconut oil, cotton seed oil, palm oil, soybean oil, and the like;alkylated vegetable oils and alkyl esters of fatty acids such asmethyloleate and the like.

Meptyl dinocap is 2,6-dinitro-4-octylphenyl crotonates and2,4-dinitro-6-octylphenyl crotonates in which ‘octyl’ is a mixture of1-methylheptyl, 1-ethylhexyl and 1-propylpentyl groups. It is a dark redviscous liquid with a pungent odour. The molecular weight of MeptylDinocap is 364.4, melting point of −22.5° C., and density of 1.10 (20°C.). The solubility of Meptyl Dinocap is water is 0.151 mg/l. It isprimarily used as a mildewicide for use on Uncinula necator in grapes inEurope (at 210 g/ha). Meptyl Dinocap is also used to control powderymildews of various crops, including pome and stone fruit, citrus,cucurbits, vegetables, tobacco, hops and ornamentals. It may secondarilycause some suppression of phytophagous mites, such as Panonychus ulmiand Hemitarsonemus latus (citrus silver mite). It is understood to referto Meptyl Dinocap per se when it is itself an oil or alternatively, theactive compound dissolved in an oil of suitable polymeric modifier.Other compounds or pesticides including fungicides, insecticides,nematocides, miticides, termiticides, rodenticides, arthropodicides,herbicides, biocides and the like may be combine with Meptyl Dinocap.Examples of such agriculturally active ingredients can be found in ThePesticide Manual, 12^(th) Edition. Exemplary pesticides which can beutilized in the oil-in-water emulsion of the present invention include,but are not limited to, benzofuranyl methylcarbamate insecticides suchas benfuracarb, and carbosulfan; oxime carbamate insecticides such asaldicarb; fumigant insecticides such as chloropicrin,1,3-dichloropropene and methyl bromide; juvenile hormone mimics such asfenoxycarb; organophosphate insecticides such as dichlorvos; aliphaticorganothiophosphate insecticides such as malathion and terbufos;aliphatic amide organothiophosphate insecticides such as dimethoate;benzotriazine organothiophosphate insecticides such as azinphos-ethyland azinphos-methyl; pyridine organothiophosphate insecticides such aschlorpyrifos and chlorpyrifos-methyl; pyrimidine organothiophosphateinsecticides such as diazinon; phenyl organothiophosphate insecticidessuch as parathion and parathion-methyl; pyrethroid ester insecticidessuch as bifenthrin, cyfluthrin, beta-cyfluthrin, cyhalothrin,gamma-cyhalothrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin,beta-cypermethrin, fenvalerate, and permethrin; and the like.

Exemplary herbicides which can be used in the oil-in-water emulsion ofthe present invention include, but are not limited to: amide herbicidessuch as dimethenamid and dimethenamid-P; anilide herbicides such aspropanil; chloroacetanilide herbicides such as acetochlor, alachlor,butachlor, metolachlor and S-metolachlor; cyclohexene oxime herbicidessuch as sethoxydim; dinitroaniline herbicides such as benfluralin,ethalfluralin, pendimethalin, and trifluralin; nitrile herbicides suchasbromoxynil octanoate; phenoxyacetic herbicides such as 4-CPA, 2,4-D,3,4-DA, MCPA, and MCPA-thioethyl; phenoxybutyric herbicides such as4-CPB, 2,4-DB, 3,4-DB, and MCPB; phenoxypropionic herbicides such ascloprop, 4-CPP, dichlorprop, dichlorprop-P, 3,4-DP, fenoprop,mecopropand mecoprop-P; aryloxyphenoxypropionic herbicides such ascyhalofop, fluazifop, fluazifop-P, haloxyfop, haloxyfop-R; pyridineherbicides such as aminopyralid, clopyralid, fluoroxypyr, picloram, andtriclopyr; triazole herbicides such as carfentrazone ethyl; and thelike.

The herbicides can also generally be employed in combination with knownherbicide safeners such as: benoxacor, cloquintocet, cyometrinil,daimuron, dichlormid, dicyclonon, dietholate, fenchlorazole,fenchlorazole-ethyl, fenclorim, flurazole, fluxofenim, furilazole,isoxadifen, isoxadifen-ethyl, mefenpyr, mefenpyr-diethyl, MG191,MON4660, R29148, mephenate, naphthalic anhydride,N-phenylsulfonylbenzoic acid amides and oxabetrinil.

Exemplary fungicides which can be used in the oil-in-water emulsion ofthe present invention include, but are not limited to, difenoconazole,dimethomorph, dinocap, diphenylamine, dodemorph, edifenphos, fenarimol,fenbuconazole, fenpropimorph, myclobutanil, oleic acid (fatty acids),propiconazole, tebuconazole and the like.

It is understood by those skilled in the art that any combination ofagriculturally active compounds may also be used in the oil-in-wateremulsion of the present invention as long as a stable and effectiveemulsion is still obtained.

The amount of Meptyl Dinocap within the oil-in-water emulsion will varydepending upon the application and the appropriate application levelswhich are well known to those skilled in the art. Typically, the totalamount of Meptyl Dinocap within the oil-in-water emulsion will be fromabout 1, generally from about 5, preferably from about 10, morepreferably from about 15 and most preferably from about 20 to about 45,generally to about 40, preferably to about 35 and most preferably toabout 30 weight percent based on the total weight of the oil-in-wateremulsion.

The polymeric modifier may be included in the oil phase to reduce thephytotoxicity of Meptyl Dinocap. The polymeric modifier also permits theuse of secondary agriculturally active ingredients that have meltingpoints below about 95 degrees Celsius. The use of the polymeric modifiermay also promote a controlled release effect of any agriculturallyactive ingredient present in the oil in water emulsion. Examples of suchagriculturally active ingredients that may be used in the oil-in-wateremulsion composition of the present disclosure include FluoroxpyrMeptyl, Chloropyrifos, Chlorpyrifos methyl, Trifluralin, Cyhalofopbutyl, Ethalfluralin, Benfluralin, Myclobutanil, Acequinocyl,Alpha-cypermethrin, Amitraz, Bensultap, Beta-cyfluthrin,Beta-cypermethrin, Bifenox, Bifenthrin, Bioresmethrin, BromoxynilOctanoate, Butralin, Cyflufenamid, Cyfluthrin, Cypermethrin,Diclofop-methyl, Dicofol, Esfenvalerate, Ethalfluralin, Etofenprox,Fenazaquin, Fenoxaprop-P-ethyl, Fenpropathrin, Fenvalerate,Flumiclorac-pentyl, Fluoroglycofen-ethyl, Flurazole, Haloxyfop-etotyl,Indoxacarb, Lambda-cyhalothrin, Metamifop, Methoxychlor, Oxyfluorfen,Pendimethalin, Permethrin, Propaquizafop, Pyributicarb,Quizalofop-P-ethyl, Trifloxystrobin, Bromophos, Fenoxaprop-ethyl,Fluazolate, Nitrofen, and Profluralin.

Suitable polymeric modifiers for addition to the oil phase arehydrophobic, have a good compatibility with Meptyl Dinocap in the oilphase, and are substantially insoluble in the aqueous phase. Examples ofsuitable polymeric modifiers may include hydrophobic modifiedcelluloses, ethyl cellulose, for example, Ethocel 10 Std FP, Ethocel Std4, Ethocel Std 7, Ethocel 45, Ethocel 100 FP, and Ethocel 300;Polyacrylate, Latex, Polycarbonate, Polyvinyl Acetate homopolymers andcopolymers, Polyolefin, Polyurethane, Polyisobutylene, Polybutene, Vinylpolymers, Polyester, Polyether, and Polyacrylonitrile.

The components of the oil-in-water emulsion are combined using a processdescribed below to produce oily globules having a lamellar liquidcrystal coating. The lamellar liquid crystal coating is an extremelyfine mono- or oligolamellar layer. Oligolamellar layer is understood torefer to a layer comprising from 2 to 5 lipid lamellae. This lamellarliquid crystal coating can be detected by Transmission ElectronicMicroscopy after cryofracture or negative stain, X-Ray diffraction orOptical Microscopy under polarized light. Terms and structure oflamellar crystal liquid phase are well defined in “The Colloidal Domain”second edition, by D. Fennell Evans and H. Wennerstrom, Wiley-VCH(1999), pages 295-296 and 306-307. The oligolamellar layer is comprisedof the non-ionic lipophilic, non-ionic hydrophilic, and ionicsurface-active agents, as stated previously. Preferably, the lipophilicsurface-active agent and the hydrophilic surface-active agent eachcontain at least one optionally saturated and/or branched fattyhydrocarbon chain having more than 12 carbon atoms, preferably from 16to 22 carbon atoms.

Preferably, the lipophilic surface-active agent has an HLB between about2 and about 5. HLB is a standard term known to those skilled in the artand refers to Hydrophilic Lipophilic Balance which identifies theemulsifier's solubility in water or oil.

Lipophilic describes the ability of a material to dissolve in a fat-likesolvent or lipid. The lipophilic surface-active agent is typicallyselected from optionally ethoxylated mono- or polyalkyl ethers or estersof glycerol or polyglycerol, mono- or polyalkyl ethers or esters ofsorbitan (optionally ethoxylated), mono- or polyalkyl ethers or estersof pentaerythritol, mono- or polyalkyl ethers or esters ofpolyoxyethylene, and mono- or polyalkyl ethers or esters of sugars.Examples of lipophilic surface-active agents include, but are notlimited to sucrose distearate, diglyceryl distearate, tetraglyceryltristearate, decaglyceryl decastearate, diglyceryl monostearate,hexaglyceryltristearate, decaglyceryl pentastearate, sorbitanmonostearate, sorbitan tristearate, diethylene glycol monostearate, theester of glycerol and palmitic and stearic acids, polyoxyethylenatedmonostearate 2 EO (containing 2 ethylene oxide units), glyceryl mono-and dibehenate and pentaerythritol tetrastearate.

Hydrophilic describes the affinity of a material to associate withwater. The hydrophilic surface-active agent typically has a HLB of fromabout 8 to about 12 and are typically selected from mono- or polyalkylethers or esters of polyethoxylated sorbitan, mono- or polyalkyl ethersor esters of polyoxyethylene, mono- or polyalkyl ethers or esters ofpolyglycerol, block copolymers of polyoxyethylene with polyoxypropyleneor polyoxybutylene, and mono- or polyalkyl ethers or esters ofoptionally ethoxylated sugars. Examples of hydrophilic surface-activeagents include, but are not limited to polyoxyethylenated sorbitanmonostearate 4 EO, polyoxyethylenated sorbitan tristearate 20 EO,polyoxyethylenated sorbitan tristearate 20 EO, polyoxyethylenatedmonostearate 8 EO, hexaglyceryl monostearate, polyoxyethylenatedmonostearate 10 EO, polyoxyethylenated distearate 12 EO andpolyoxyethylenated methylglucose distearate 20 EO.

In addition to the lipophilic and hydrophilic surface-active agents, anionic surface-active agent also comprises the oligolamellar layer of thelamellar liquid crystal coating.

Ionic surface-active agents which can be used in the oil-in-wateremulsion of the present invention include (a) neutralized anionicsurface-active agents, (b) amphoteric surface-active agents, (c)alkylsulphonic derivatives and (d) cationic surface-active agents.

Neutralized anionic surface-active agents (a) include, but are notlimited to, for example:

-   -   alkali metal salts of dicetyl phosphate and dimyristyl        phosphate, in particular sodium and potassium salts;    -   alkali metal salts of cholesteryl sulphate and cholesteryl        phosphate, especially the sodium salts;    -   lipoamino acids and their salts, such as mono- and disodium        acylglutamates, such as the disodium salt of        N-stearoyl-L-glutamic acid, the sodium salts of phosphatidic        acid;    -   phospholipids; and    -   the mono- and disodium salts of acylglutamic acids, in        particular N-stearoylglutamic acid.

Anionic surface-active agents chosen from alkyl ether citrates andmixtures thereof which can be used in the oil-in-water emulsions of thepresent invention are disclosed in U.S. Pat. No. 6,413,527, which isincorporated herein by reference. Alkyl ether citrates includemonoesters or diesters formed by citric acid and at least oneoxyethylenated fatty alcohol comprising a saturated or unsaturated,linear or branched alkyl chain having from 8 to 22 carbon atoms andcomprising from 3 to 9 oxyethylene groups, and mixtures thereof. Thesecitrates can be chosen, for example from the mono- and diesters ofcitric acid and of ethoxylated lauryl alcohol comprising from 3 to 9oxyethylene groups. The alkyl ether citrates are preferably employed inthe neutralized form at a pH of about 7. Neutralization agents can beingchosen from inorganic bases, such as sodium hydroxide, potassiumhydroxide or ammonia, and organic bases, such as mono-, di- andtriethanolamine, aminomethyl-1,3-propanediol, N-methylglucamine, basicamino acids, such as arginine and lysine and mixtures thereof.

Amphoteric surface-active agents (b) include, but are not limited tophospholipids and especially phosphatidylethanolamine from pure soya.

Alkylsulphonic derivatives (c) include, but are not limited to compoundsof the formula:

in which R represents the radicals C₁₆H₃₃ and C₁₈H₃₇, taken as a mixtureor separately, and M is an alkali metal, preferably sodium.

Cationic surface-active agents (d) include but are not limited tosurface-active agents as disclosed in U.S. Pat. No. 6,464,990, which isincorporated herein by reference. They are typically selected from thegroup of quaternary ammonium salts, fatty amines and salts thereof. Thequaternary ammonium salts include, for example: those which exhibit thefollowing formula:

wherein the R1 to R4 radicals, which can be identical or different,represent a linear or branched aliphatic radical comprising from 1 to 30carbon atoms or an aromatic radical, such as aryl or alkylaryl. Thealiphatic radicals can comprise heteroatoms, such as oxygen, nitrogen,sulfur and halogens. The aliphatic radicals include alkyl, alkoxy,polyoxy(C₂-C₆)alkylene, alkylamido, (C₁₂-C₂₂)alkylamido(C₂-C6) alkyl,(C₁₂-C₂₂)alkyl acetate and hydroxyalkyl radicals comprisingapproximately from 1 to 30 carbon atoms; X is an anion selected fromhalides, phosphates, acetates, lactates, (C₂-C₆)alkyl sulfates, andalkyl- or alkylarylsulfonates. Preference is given, as quaternaryammonium salts to tetraalkylammonium chlorides, such asdialkyldimethylammonium and alkyltrimethylammonium chlorides in whichthe alkyl radical comprises approximately from 12 to 22 carbon atoms, inparticularly behenyltrimethylammonium, distearyldimethylammonium,cetyltrimethylammonium and benzyldimethylstearylammonium chlorides, oralternatively, stearamidopropyl-dimethyl(myristyl acetate) ammoniumchloride; imidazolinium quaternary ammonium salts, such as those offormula:

wherein R5 represents an alkenyl or alkyl radical comprising from 8 to30 carbon atoms, for example derived from tallow fatty acids; R6represents a hydrogen atom, an alkyl radical comprising from 1 to 4carbon atoms or an alkenyl or alkyl radical comprising from 8 to 30carbon atoms; R7 represents an alkyl radical comprising from 1 to 4carbon atoms; R8 represents a hydrogen atom or an alkyl radicalcomprising from 1 to 4 carbon atoms; and X is an anion selected from thegroup of the halides, phosphates, acetates, lactates, alkyl sulfates, oralkyl, and alkylarylsulfonates. R5 and R6 preferably denote a mixture ofalkenyl or alkyl radicals comprising from 12 to 21 carbon atoms, forexample derived from tallow fatty acids, R7 preferably denotes a methylradical and R8 preferably denotes hydrogen. Quaternary diammonium saltsare also contemplated, such as propanetallowediammonium dichloride.

Fatty amines include, but are not limited to those of formula:R9(CONH)_(n)(CH₂)_(m)N(R11)R10wherein R9 is an optionally saturated and/or branched hydrocarbon chain,having between 8 and 30 carbon atoms, preferably between 10 and 24carbon atoms; R10 and R11 are selected from H and an optionallysaturated and/or branched hydrocarbon chain, having between 1 and 10carbon atoms; preferably between 1 and 4 carbon atoms; m is an integerbetween 1 and 10 and is preferably between 1 and 5; and n is either 0 or1.

Examples of fatty amines include, but are not limited to, stearylamine,aminoethyl-ethanolamide stearate, diethylenetriamine stearate,palmitamidopropyldimethyl-amine, palmitamidopropyldiethylamine,palmitamidoethyldiethylamine, palmitamidoethyldimethylamine.Commercially available fatty amines include, but are not limited to,Incromine™ BB from Croda, Amidoamine™ MSP from Nikkol, and Lexamine™series from Inolex, the Acetamine series from Kao Corp; Berol 380, 390,453 and 455, and Ethomeen™ series from Akzo Nobel, and Marlazin™ L10,OL2, OL20, T15/2, T50 from Condea Chemie.

As described above, the surface-active agents form the lamellar liquidcrystal coating of the oily globules suspended within the aqueous phaseof the oil-in-water emulsion of the present invention. The amount of thethree surface-active agents utilized in the oil-in-water emulsion of thepresent invention is typically from about 20, preferably from about 35to about 65, preferably to about 55 weight percent of non-ioniclipophilic surface-active agent, from about 15, preferably from about 25to about 50, preferably to about 40 weight percent of non-ionichydrophilic surface-active agent and from about 5, preferably from about10 to about 45, preferably to about 35 weight percent of ionicsurface-active agent; based on the total combined weight of surfaceactive agents. The coating of the oily globules comprises a total amountof hydrophilic surface-active agent, lipophilic surface-active agent andionic surface-active agent to be between about 2 and about 20 percent byweight, based on the total weight of the oil-in-water emulsion.Preferably the total amount is from about 2.5, more preferably fromabout 3 to 10, more preferably to about 6 weight percent, based on thetotal weight of the oil-in-water emulsion.

The ratio of the total weight of the surface-active compounds to thetotal weight of oil is typically from 1:2.5 to 1:25.

The amount of the polymeric modifier utilized in the oil-in-wateremulsion of the present disclosure is typically from about 0.1,preferably from about 1 to about 30, most preferably from 2 to 20 weightpercent based on the total weight of the oil-in-water emulsion.

The aqueous phase is typically water, for example, deionized water. Theaqueous phase may also contain other additives such as compounds thatlower the freezing point, for example alcohols, e.g. isopropyl alcoholand propylene glycol; pH buffering agents, for example alkali phosphatessuch as sodium phosphate monobasic monohydrate, sodium phosphatedibasic; biocides, for example Proxel GXL; and antifoams, for exampleoctamethylcyclotetrasiloxane (Antifoam A from Dow Corning). Otheradditives and/or adjuvants can also be present in the aqueous phase aslong as the stability of the oil-in-water emulsion is still maintained.Other additives also include water-soluble agriculturally activecompounds.

The oil phase or the coated oily globules are from 5, preferably from 8and more preferably from 10 to 50 percent, preferably to 45 and mostpreferably to 40 weight percent, based on the total weight of theoil-in-water emulsion composition. The oil/water ratio is typically lessthan or equal to 1.

Other additives and/or adjuvants can also be present within theoil-in-water emulsion of the present invention, as long as the stabilityand activity of the oil-in-water emulsion is still obtained. Theoil-in-water emulsions of the present invention may additionally containadjuvant surface-active agents to enhance deposition, wetting andpenetration of the agriculturally active ingredient onto the targetsite, e.g. crop, weed or organism. These adjuvant surface-active agentsmay optionally be employed as a component of the emulsion in either theoil or water phase, or as a tank mix component; the use of and amountdesired being well known by those skilled in the art. Suitable adjuvantsurface-active agents include, but are not limited to ethoxylated nonylphenols, ethoxylated synthetic or natural alcohols, salts of the estersor sulphosuccinic acids, ethoxylated organosilicones, ethoxylated fattyamines and blends of surface-active agents with mineral or vegetableoils.

The oil-in-water emulsion of the present invention can be preparedaccording to the process described in U.S. Pat. No. 5,925,364, theteachings of which are incorporated herein by reference. Theagriculturally active ingredient or a combination of agriculturallyactive ingredients is first melted or dissolved into the polymericmodifier, adding solvent if desired, after which the nonionicsurface-active agent(s) is dissolved into the mixture. The then mixtureis homogenized by cavitation using a high pressure homogenizer, toprovide the small particle sized oily globules. The mean size of thecoated oily globules is typically less than 800 nanometers, preferablyless than 500 nanometers and most preferably about 200 nanometers, asdetermined using laser diffraction particle size analysis and scanningelectron microscopy.

In one embodiment, the oil-in-water emulsion is prepared by:

-   -   1) dissolving polymeric modifier and any other agriculturally        active ingredient(s) into Meptyl Dinocap and optionally a        suitable solvent;    -   2) mixing an oil phase, comprising the lipophilic surfactant,        the polymeric modifier, Meptyl Dinocap, the hydrophilic        surfactant, and optionally a suitable solvent and (B) an aqueous        phase containing ionic surfactant to obtain a mixture; and    -   3) homogenizing the mixture by subjecting the mixture to        cavitation.

In the first step, the mixture can be formed by conventional stirring,for example, using a high shear homogenizer rotating at a rate ofapproximately between 2000 and 7000 rpm for a time approximately between5 and 60 minutes and at a temperature between approximately 20° C. and95° C.

The homogenization can be performed by using a high pressure homogenizeroperating at pressures between approximately 200 and 1000 bar as is wellknown to those skilled in the art. The process is performed bysuccessive passages, generally from 1 to 12 passages, at a selectedpressure; the mixture being returned to normal pressure between eachpassage. The homogenization of the second step may also be carried outunder the action of ultrasound or alternatively by the use of ahomogenizer equipped with a rotor-stator type head.

Another embodiment of the present invention is the use of theoil-in-water emulsion in agricultural applications to control, preventor eliminate unwanted living organisms, e.g. fungi, weeds, insects,bacteria or other microorganisms and other pests. This would includeits' use for protection of a plant against attack by a phytopathogenicorganism or the treatment of a plant already infested by aphytopathogenic organism, comprising applying the oil-in-water emulsioncomposition, to soil, a plant, a part of a plant, foliage, flowers,fruit, and/or seeds in a disease inhibiting and phytologicallyacceptable amount. The term “disease inhibiting and phytologicallyacceptable amount” refers to an amount of a compound that kills orinhibits the plant disease for which control is desired, but is notsignificantly toxic to the plant. The exact concentration of activecompound required varies with the fungal disease to be controlled, thetype of formulations employed, the method of application, the particularplant species, climate conditions, and the like, as is well known in theart.

Additionally, the oil-in-water emulsions of the present invention areuseful for the control of insects or other pests, e.g. rodents.Therefore, the present invention also is directed to a method forinhibiting an insect or pest which comprises applying to a locus of theinsect or pest an oil-in-water emulsion comprising an insect-inhibitingamount of an agriculturally active compound for such use. The “locus” ofinsects or pests is a term used herein to refer to the environment inwhich the insects or pests live or where their eggs are present,including the air surrounding them, the food they eat, or objects whichthey contact. For example, insects which eat or contact edible orornamental plants can be controlled by applying the active compound toplant parts such as the seed, seedling, or cutting which is planted, theleaves, stems, fruits, grain, or roots, or to the soil in which theroots are growing. It is contemplated that the agriculturally activecompounds and oil-in-water emulsions containing such, might also beuseful to protect textiles, paper, stored grain, seeds, domesticatedanimals, buildings or human beings by applying an active compound to ornear such objects. The term “inhibiting an insect or pest” refers to adecrease in the numbers of living insects or pests, or a decrease in thenumber of viable insect eggs. The extent of reduction accomplished by acompound depends, of course, upon the application rate of the compound,the particular compound used, and the target insect or pest species. Atleast an inactivating amount should be used. The terms “insect orpest-inactivating amount” are used to describe the amount, which issufficient to cause a measurable reduction in the treated insect or pestpopulation, as is well known in the art.

The locus to which a compound or composition is applied can be any locusinhabited by an insect, mite or pest, for example, vegetable crops,fruit and nut trees, grape vines, ornamental plants, domesticatedanimals, the interior or exterior surfaces of buildings, and the soilaround buildings.

Because of the unique ability of insect eggs to resist toxicant action,repeated applications may be desirable to control newly emerged larvae,as is true of other known insecticides and acaricides.

Additionally, the present invention relates to the use of oil-in-wateremulsions comprising agriculturally active compounds which areherbicides. The term herbicide is used herein to mean an activeingredient that kills, controls or otherwise adversely modifies thegrowth of plants. An herbicidally effective or vegetation controllingamount is an amount of active ingredient which causes an adverselymodifying effect and includes deviations from natural development,killing, regulation, desiccation, retardation, and the like. The termsplants and vegetation include emerging seedlings and establishedvegetation.

Herbicidal activity is exhibited when they are applied directly to thelocus of the undesirable plant thereof at any stage of growth or beforeemergence of the weeds. The effect observed depends upon the plantspecies to be controlled, the stage of growth of the plant, the particlesize of solid components, the environmental conditions at the time ofuse, the specific adjuvants and carriers employed, the soil type, andthe like, as well as the amount of chemical applied. These and otherfactors can be adjusted as is known in the art to promote selectiveherbicidal action. Generally, it is preferred to apply such herbicidespost emergence to relatively immature undesirable vegetation to achievethe maximum control of weeds.

Another specific aspect of the present invention is a method ofpreventing or controlling pests such as nematodes, mites, arthropods,rodents, termites, bacteria or other microorganisms, comprising applyingto a locus where control or prevention is desired a composition of thepresent invention which comprises the appropriate active compound suchas a nematocide, miticide, arthropodicide, rodenticide, termiticide orbiocide.

The actual amount of agriculturally active compound to be applied toloci of disease, insects and mites, weeds or other pests is well knownin the art and can readily be determined by those skilled in the art inview of the teachings above.

The composition of the present invention surprisingly offers stableagricultural oil-in-water emulsions having low viscosity and long termshelf life. Additionally, the stable agricultural oil-in-water emulsionsof the present invention can offer other surprising improvements, e.g.efficacy.

The following examples are provided to illustrate the present invention.The examples are not intended to limit the scope of the presentinvention and they should not be so interpreted. Amounts are in weightparts or weight percentages unless otherwise indicated.

EXAMPLES

These examples are provided to further illustrate the invention and arenot meant to be construed as limiting.

As disclosed herein, all temperatures are given in degrees Celsius andall percentages are weight percentages unless otherwise stated. Theactive ingredients in all examples are super-saturated.

In these examples, the process is performed using the followingprocedure:

The polymeric modifier is first dissolved into the oil phase Acontaining Meptyl Dinocap, optional a solvent and surfactants. The oilphase A and the aqueous phase B containing ionic surfactant are heatedseparately to the desired temperature. Phase A is poured into Phase B,with stirring of 4000-8000 rpm provided by a Silverson L4RT high shearhomogenizer fitted with a square hole high shear screen. Stirring andtemperature conditions are maintained for 10 minutes.

The mixture is then introduced into a Niro Soavi high pressure 2-stagehomogenizer of type Panda 2K, which is adjusted to a pressure of 1000bar for 1 to 12 successive passages.

A stabilized oil-in-water emulsion is thus obtained, the oily globulesof which have a mean diameter of typically around 200-300 nm.

Example 1

Table 1, shown below, illustrates an oil-in-water emulsion of thepresent invention that includes Meptyl Dinocap and a polymeric modifier.Table 2, shown below, illustrates comparative greenhouse phytotoxicitydata for grapes sprayed with the described compounds. The grapes weresprayed with 2.5 ml of the designated mixtures at 35° C. with spray rateat 1×/2× of field rate (field rate: 210 gai/hl) and evaluated after 24hours to determine if the plant was damaged by the respective compounds.Phytotoxicity was measured as a percentage of total tissue with damage.As shown in Table 2, the formulation illustrated by Table 1 producedcomparatively low phytotoxicity in the plants. Here, GF-1138(Karathane™) is a 350 g/l Dinocap EC in aromatic solvent; GF-1478 is asimilar 350 g/l EC in aromatic solvent; GF-1804 is a 350 g/loil-in-water emulsion without polymer added; GF-1675 is a 250 g/loil-in-water emulsion without polymer added.

TABLE 1 Meptyl Dinocap oil-in-water emulsion with 2.59% Ethocel STD 10Meptyl Dinocap 26.30 Ethocel std 10 2.59 Brij 72 2.65 Sorbitan (40EO)stearate (Tween 61 by Uniqema) 1.85 Oil (N,N-dimethyl fatty acid amide)10.00 Aqueous Phase B Deionized water 46.11 Propylene Glycol 10.00Amisoft HS-21P 0.50

TABLE 2 Comparison of Meptyl Dinocap greenhouse phytotoxicity data Ratecompared % Phytotoxicity Formulation to field rate (35° C.) GF-1138 (350g/l EC) 2× 59 1× 10 GF1478 (350 g/l EC) 2× 8 1× 18 GF1804 (350 g/loil-in-water emulsion) 2× 64 1× 9 GF1675 (250 g/l oil-in-water emulsion)2× 41 1× 19 Oil-in-water emulsion from 2× 3 Table 1 with 2.59% 1× 0Ethocel (mean particle size 224 nm) untreated 0 0

The Meptyl Dinocap oil-in-water emulsion with Ethocel was evaluated forbiological efficacy versus an EC formulation (GF1478). Results werelisted in Table 3. It was found that the Meptyl Dinocap oil-in-wateremulsion with Ethocel demonstrated equivalent efficacy to EC formulation(GF1478).

TABLE 3 Efficacy of oil-in-water emulsions with 2.59% Ethocel and 1.5%Ethocel % Disease with UNCINE on grape Rate compared to Emulsion withEmulsion with field rate GF1478 (EC) 2.59% Ethocel 1.5% Ethocel 0.5× 00.25 0 0.125× 1.25 0.25 0 0.03× 2 3.25 4.75 0.01× 5.75 5.25 4.25 (1 dayprotectant test; high volume; 12 days post inoculation)

Example 2

In Table 4, shown below, all of the described samples have the sameformulation as described above. The only difference is the oil dropletsize has been changed by varying the total number of homogenizationpasses. It was found that the phytotoxicity performance with the currentinvention is independent of oil droplet size.

TABLE 4 Phytotoxicity performance of Meptyl Dinocap oil-in-wateremulsion with 2.59% Ethocel STD 10 versus mean oil droplet size Mean oildroplet size Rate compared % Phytotoxicity (um) to field rate 35° C.32.86 2× 0 1× 0 0.302 2× 2 1× 0 0.240 2× 0 1× 2 0.224 2× 3 1× 0

Example 3

Table 5, shown below, illustrates the results of an experiment comparingphytotoxicity performance and solvent concentration. For this example,all of the samples have the same formulation as described above with theexception that the solvent (N,N-dimethyl fatty acid amide) concentrationwas varied. In addition, two different levels of Ethocel STD 10, thepolymeric modifier, were tested. It was found that the phytotoxicityperformance with the current invention increases slightly with increasedsolvent level.

TABLE 5 Phytotoxicity performance of Meptyl Dinocap oil-in-waterEmulsions with Ethocel STD 10 versus solvent concentration (N,N-dimethylfatty acid amide) Rate compared % Phytotoxicity Formulation to fieldrate (35° C.) Emulsion with 2.59% 2× 12 Ethocel STD 10 and 1× 2 15%N,N-dimethyl fatty acid amide Emulsion with 2.59% 2× 3 Ethocel STD 10and 1× 0 10% N,N-dimethyl fatty acid amide Emulsion with 1.5% 2× 18Ethocel STD 10 and 1× 0 15% N,N-dimethyl fatty acid amide EcoZome with1.5% 2× 7 Ethocel STD 10 and 1× 0 10% N,N-dimethyl fatty acid amide

What is claimed is:
 1. An oil-in-water emulsion composition having anoil phase and water phase, the oil phase comprising: a polymericmodifier, wherein the polymeric modifier is dissolved in the oil phase,and wherein the oil phase exhibits a lamellar liquid crystal coating; anagriculturally active ingredient including Meptyl Dinocap; at least onenon-ionic lipophilic surface-active agent; at least one non-ionichydrophilic surface-active agent; at least one ionic surface-activeagent.
 2. The composition of claim 1 wherein the non-ionic lipophilicsurface-active agent has an Hydrophilic Lipophilic Balance of between 2and
 5. 3. The composition of claim 2, wherein the non-ionic lipophilicsurface-active agent is selected from the group consisting of optionallyethoxylated mono- or polyalkyl ethers or esters of glycerol orpolyglycerol, optionally ethoxylated mono- or polyalkyl ethers or estersof sorbitan, mono- or polyalkyl ethers or esters of pentaerythritol,mono- or polyalkyl ethers or esters of polyoxyethylene, and mono- orpolyalkyl ethers or esters of sugars.
 4. The composition of claim 3,wherein the non-ionic lipophilic surface-active agent is selected fromthe group consisting of sucrose distearate, diglyceryl distearate,tetraglyceryl tristearate, decaglyceryl decastearate, diglycerylmonostearate, hexaglyceryltristearate, decaglyceryl pentastearate,sorbitan monostearate, sorbitan tristearate, diethylene glycolmonostearate, the ester of glycerol and palmitic and stearic acids,polyoxyethylenated monostearate 2 EO (containing 2 ethylene oxideunits), glyceryl mono- and dibehenate and pentaerythritol tetrastearate.5. The composition of claim 1, wherein the non-ionic hydrophilicsurface-active agent has an Hydrophilic Lipophilic Balance between 8 and12.
 6. The composition of claim 5, wherein the non-ionic hydrophilicsurface-active agent is selected from the group consisting of mono- orpolyalkyl ethers or esters of polyethoxylated sorbitan, mono- orpolyalkyl ethers or esters of polyoxyethylene, mono- or polyalkyl ethersor esters of polyglycerol, block copolymers of polyoxyethylene withpolyoxypropylene or polyoxybutylene, and mono- or polyalkyl ethers oresters of optionally ethoxylated sugars.
 7. The composition of claim 6,wherein the non-ionic hydrophilic surface-active agent is selected fromthe group consisting of polyoxyethylenated sorbitan monostearate 4 EO,polyoxyethylenated sorbitan tristearate 20 EO, polyoxyethylenatedsorbitan tristearate 20 EO, polyoxyethylenated monostearate 8 EO,hexaglyceryl monostearate, polyoxyethylenated monostearate 10 EO,polyoxyethylenated distearate 12 EO and polyoxyethylenated methylglucosedistearate 20 EO.
 8. The composition of claim 1, wherein the ionicsurface-active agent is selected from the group consisting of (a)neutralized anionic surface-active agents, (b) amphoteric surface-activeagents, (c) alkylsulphonic derivatives and (d) cationic surface-activeagents.
 9. The composition of claim 8, wherein the ionic surface-activeagent is selected from the group consisting of: alkali metal salts ofdicetyl phosphate and dimyristyl phosphate; alkali metal salts ofcholesteryl sulphate and cholesteryl phosphate; lipoamino acids,lipoamino acids acylglutamates, sodium salts of phosphatidic acid;phospholipids; and alkyl ether citrates.
 10. The composition of claim 8,wherein the ionic surface-active agent is a phospholipid.
 11. Thecomposition of claim 8, wherein the ionic surface-active agent is analkylsulphonic derivative.
 12. The composition of claim 8, wherein theionic surface-active agent is selected from the group consisting ofquaternary ammonium salts, fatty amines and salts thereof.
 13. Thecomposition of claim 1, further comprising another agriculturally activecompound selected from the group consisting of fungicides, insecticides,nematocides, miticides, biocides, termiticides, rodenticides,arthropodicides, and herbicides.
 14. A method of controlling orpreventing fungal attack, comprising applying a composition of claim 13to the fungus, soil, plant, root, foliage, seed or locus in which theinfestation is to be prevented or controlled.
 15. The composition ofclaim 1, wherein the polymeric modifier is selected from the groupconsisting of ethyl cellulose resins, Polycarbonate, Polyvinyl acetatehomopolymers and copolymers, Polyolefin, Polyurethane, Polyisobutylene,Polybutene, vinyle polymers, Polyester, Polyether, andPolyacrylonnitrile.
 16. The composition of claim 1, wherein theoil-in-water emulsion composition is from about 1 to about 60 weightpercent total oil phase, from about 0.2 to about 30 weight percentpolymeric modifier, from about 1 to about 45 weight percentagriculturally active compound, from about 0.4 to about 13 weightpercent non-ionic lipophilic surface-active agent, from about 0.3 toabout 10 weight percent non-ionic hydrophilic surface-active agent, fromabout 0.1 to about 9 weight percent ionic surface-active agent, based ona total weight of the oil-in-water emulsion composition.
 17. Thecomposition of claim 9, wherein the metal in the alkali metal salts ofdicetyl phosphate and dimyristyl phosphate is either sodium orpotassium.
 18. The composition of claim 9, wherein the metal in thealkali metal salts of cholesteryl sulphate and cholesteryl phosphate issodium.
 19. The composition of claim 9, wherein the lipoamino acid isthe disodium salt of N-stearoyl-L-glutamic acid.
 20. The composition ofclaim 9, wherein the acylglutamic acid is N-stearoylglutamic acid.